Yes, Kithara DOS Enabler enables hardware-dependent DOS and 16 bit Windows programs to work under Windows 10.
32-bit and 64-bit systems are supported.
Kithara DOS Enabler has its own DOS-System (FreeDOS) and executes the 16-bit applications native on a dedicated CPU core.
By the elimination of any impacts of the Windows system time-critical applications are possible.

For programming hardware-dependent and time-critical 32-bit and 64-bit applications see Kithara RealTime Suite.