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It is possible to use real-time on a Windows 10 x64 systems?

Yes. However, Windows 10 x64 has the so called 'Kernel Patch Protection' in order to restrain access to the OS. Thus, the code execution of real-time tasks on CPU cores, which are also used by Windows, is not possible.

Nevertheless, by using the Dedicated Module it is possible to keep single or multi CPU cores free by the Windows OS (further information). In this case, the Kithara real-time system is booted exclusively on the respective CPUs. That enables real-time on a Windows 10 x64 systems with the best real-time features.